NEBNext Direct technology will initially focus on marker-assisted breeding

crop development

NEBNext Direct is a new technology that will facilitate high-throughput molecular breeding for crop development (Credit: freestocks-photos from Pixabay)

New England Biolabs (NEB), a provider of enzymes for molecular biology applications, is set to unveil new solutions to support agricultural biology.

NEB will conduct a workshop to show the latest advances in next-generation sequencing (NGS) sample preparation at the upcoming Plant and Animal Genome (PAG) Meeting, which is being held in San Diego, California.

At the event, the company will exhibit novel products for DNA and RNA sample preparation, including the NEBNext Direct genotyping solution.

NEBNext Direct will support high-throughput molecular breeding for crop development

NEBNext Direct is a new technology designed to deliver high-throughput molecular breeding for crop development.

The new genotyping solution helps to implement a novel NGS-based targeted genotyping approach that will help to control cost and improve throughput.

The new technology, which initially focuses on marker-assisted breeding, can make a genotyping between 100 and 5,000 markers in parallel by using a capture-based enrichment approach.

NEB senior application specialist Dr Bjoern Textor said: “Genotyping for molecular breeding requires solutions that can support robust processes in order to implement at scale.

“The NEBNext Direct Genotyping Solution has intrinsic features facilitating high-throughput sample processing, including pre-capture barcoding, pooling of up to 96 samples per capture, and dual indexing, allowing up to 9,216 samples to be sequenced in parallel on the Illumina platform.”

NEB has partnered with Beckman Coulter Life Sciences and the European Molecular Biology Laboratory (EMBL) Genomics Core Facility (GeneCore) in Heidelberg for the development of an automated protocol.

GeneCore senior automation engineer Dr Juergen Zimmermann said: “The chemistry, superior performance and stability of the NEBNext Direct Genotyping Solution combined with the flexibility of the Biomek i7 Workstation provides an excellent solution for building scalable, highly integrated and automated environments for full walkaway processes.

“The intelligent utilisation of standard plastics combined with optimized chemistry consumption makes it an optimal tool for various applications in different areas.”